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Enzyme-linked thyroglobulin immunoassay
Rev. Hosp. Säo Paulo Esc. Paul. Med ; 6(3/4): 87-90, July-Dec. 1995. ilus, graf
Article Dans Anglais | LILACS | ID: lil-180111
ABSTRACT
A one-step enzyme linked sandwich immunoassay using Silicone rods coated with rabbit anti-human thyroglobulin anti-Tg) immunoglobulin G (Fab') conjugated with beta-D-galactosidase was established for the measurement of thyroglobulin in human sera. The volume of serum needed for the assay was 2 mul The sensitivity of the assay was 1.52 amol/tube, corresponding to O.5 ng/ml. The precision was proven by coefficients of variation intra-assay, 7.0 to 9.1 per cent inter-assay, 5.3 to 7.4 per cent. The correlation between this EIA and RIA was O.91, p < O.O1.
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Indice: LILAS (Amériques) Sujet Principal: Autoanticorps / Thyroglobuline / Immunoglobuline G / Beta-Galactosidase Type d'étude: Etude diagnostique Limites du sujet: Animaux / Humains langue: Anglais Texte intégral: Rev. Hosp. Säo Paulo Esc. Paul. Med Thème du journal: Médicament Année: 1995 Type: Article

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Indice: LILAS (Amériques) Sujet Principal: Autoanticorps / Thyroglobuline / Immunoglobuline G / Beta-Galactosidase Type d'étude: Etude diagnostique Limites du sujet: Animaux / Humains langue: Anglais Texte intégral: Rev. Hosp. Säo Paulo Esc. Paul. Med Thème du journal: Médicament Année: 1995 Type: Article