Biolistic-mediated gene expression in guinea pigs and cattle tissues in vivo
Braz. j. med. biol. res
; 29(10): 1265-7, Oct. 1996. ilus, tab
Article
Dans En
| LILACS
| ID: lil-186172
Responsable en Bibliothèque :
BR1.1
ABSTRACT
Foreign genes were introduced and expressed in vivo in guinea pigs and cattle utilizing a new hand-held device based on high-pressure helium gas to accelerate DNA-coated microparticles. Guinea pigs were used to evaluate the physical parameters to introduce and express the exogenous DNA. The best conditions were applied to conduct bombardments in cattle. The results showed a high frequency of gene expression in all the bombarded cattle. This procedure could be used to study the immune responses in cattle and in a wide variety of animals through genetic immunization.
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Indice:
LILACS
Sujet Principal:
Expression des gènes
/
Beta-Galactosidase
/
Biolistique
Limites du sujet:
Animals
langue:
En
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
BIOLOGIA
/
MEDICINA
Année:
1996
Type:
Article