Age-dependent morphological changes in dBcAMP-treated astrocytes
Medicina (B.Aires)
;
56(4): 389-92, 1996. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-186261
ABSTRACT
Since changes in cell morphology are conspicuous features of astrocyte reaction, we resorted to an histometric approach to evaluate age influence on such morphological response to activating stimuli. To this end, first subculture of rat brain astrocytes at 1, 9 or 21 days in vitro (DIV) were treated during 2 hs with l mM of dBcAMP, a chemical compound known to induce cell differentiation. Following treatment, immunoperoxidase labeling of GFAP, specific marker of astrocyte activation, was carried out. Although total count of GFAP-positive cell foci was greater in treated samples in all times tested, when such cell foci were evaluated by image analysis, differences between perimeter/area ratios of such foci were only statistifically significant at l DIV. It may be concluded that while dBcAMP effect is maintained despite astrocyte aging, the morphological pattern of response varies markedly along the observation period.
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Indice:
LILAS (Amériques)
Sujet Principal:
Différenciation cellulaire
/
Astrocytes
/
Dibutyryl AMP cyclique
/
Cerveau
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Medicina (B.Aires)
Thème du journal:
Médicament
Année:
1996
Type:
Article
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