Identification and neutralization of biological activities in the venoms of Loxosceles spiders
Braz. j. med. biol. res
;
29(11): 1491-7, Nov. 1996. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-187211
ABSTRACT
The biological activities of the venom of three species of spiders of the genus Loxosceles were studied (L. gaucho, L. laeta and L. intermedia). The dermonecrotic and lethal activities are shared by all three Loxosceles venoms. Only low levels of proteolytic, myotoxic and phospholipase A2 activities were demonstrable even when a large amount of venom was used. No direct hemolytic activitiy was detected. L. intermedia venom was the most lethal (LD50 0.48 mg/kg), the L. laeta venom was the least lethal (LD50 1.45 mg/kg) whereas L. gaucho venom showed an intermediate value (LD50 0.74 mg/kg). The anti-Loxosceles serum used (anti-arachnidic serum) was able to neutralize the most important activities (i.e., dermonecrotic and lethal activities) of the three venoms. SDS-PAGE and immunoblotting using the anti-arachnidic serum showed that almost all venom antigens were recognized by this antiserum. The possible mechanisms of action of the Loxosceles venom are discussed.
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Indice:
LILAS (Amériques)
Sujet Principal:
Venins d'araignée
/
Araignées
/
Sérums immuns
/
Nécrose
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1996
Type:
Article
/
descriptif de projet
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