Diameter of the mammalian porin channel in open and "closed" states: direct measurement at the single channel level in planar lipid bilayer
Braz. j. med. biol. res
;
29(12): 1691-7, Dec. 1996. graf
Article
Dans Anglais
| LILACS
| ID: lil-188456
RESUMO
Porin isolated from bovine skeletal muscle was reconstituted in planar lipid bilayers under voltage clamp conditions. A set of non-electrolytes were used as molecular probes for determining the pore diameter. The maximal diameter of the open channel was estimated to be 3.02 + 0.26 nm. As observed for other porin channels, a large transmembrane potential drove the channel into a "closed" state. The channel transition to the low conductance (closed) state was followed by a decrease in the maximal diameter of the channel to 2.4 +- 0.08 nm.
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Indice:
LILAS (Amériques)
Sujet Principal:
Techniques in vitro
/
Porines
/
Canaux ioniques
/
Double couche lipidique
/
Mammifères
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1996
Type:
Article
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