Proteolytic and partial characterization of human sperm-surface glycopeptides
Braz. j. med. biol. res
;
30(3): 387-93, Mar. 1997. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-191350
RESUMO
Sperm-surface glycopeptides were obtained from intact sperm membranes after proteolytic release by different enzymatic treatments such as autoproteolysis, trypsin, papain and pronase. Glycopeptides were isolated, their properties and composition were examined, and their monosaccharide and amino acid constituents were characterized. The monosaccharides identified were fucose, mannose, galactose, N-acetylglucosamine, and N-acetylgalactosamine, which form part of more than one type of oligosaccharide units. Autoproteolytic treatmentmainly provided O-glycosidic type oligosaccharides, while a mixture of O- and N-glycosidic oligosaccharides was obtained in variable proportions when treated with trypsin, papain or pronase. The highest degree of peptide cleavage was obtained with pronase. Despite the higher yields reached with trypsin, these glycopeptides contain the lowest percentage of oligosaccharide chains. Proteolytic treatment provides a simple, rapid procedure for the isolation of glycopeptides from the sperm surface.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Peptide hydrolases
/
Spermatozoïdes
/
Glycopeptides
/
Papaïne
/
Pronase
/
Trypsine
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1997
Type:
Article
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