Cloning and characterization of a Schistosoma mansoni cDNA clone with a specific antigenic expression during development in a vertebrate host
Mem. Inst. Oswaldo Cruz
;
92(5): 643-6, Sept.-Oct. 1997. ilus
Article
Dans Anglais
| LILACS
| ID: lil-194208
ABSTRACT
Approximately 2.0 x 10 cDNA clones of an Schistosoma mansoni gt11 cDNA library were screened in duplicate with serum from infected mice corresponding to distinct phases of infection. A cDNA clone (7/1) was isolated and recognized only by seven week serum. The clone was subcloned in pGEX-2T and Western-blot studies showed a specific antigenic expression confirming that only serum from the chronic phase is capable of recognizing this antigen. Dot-hybridization with RNA from different developmental phases of the parasite showed that the corresponding 7/1 RNA is expressed in all phases of parasite development in vertebrate hosts.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Schistosoma mansoni
/
Clonage moléculaire
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1997
Type:
Article
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