The Trypanosoma cruzi genome project: nuclear karyotype and gene mapping of clone CL Brener
Mem. Inst. Oswaldo Cruz
;
92(6): 821-8, Nov.-Dec. 1997. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-197223
ABSTRACT
By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference chromosomal bands ranging in size from 0.45 to 3.5 Magabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma cruzi
/
Cartographie chromosomique
/
Clones cellulaires
/
Caryotypage
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1997
Type:
Article
/
Congrès et conférence
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