Flow cytometry evaluation of complement mediated bacterial membrane damage
Arch. med. res
;
27(4): 459-63, 1996. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-200348
RESUMO
The study of the complement-outer bacterial membrane interactions is gaining enthusiasm for applying a continous development of more specialized techniques. In this paper a novel flow cytometry technique shows that highly fluorescent lucifer yellow-stained Neisseriae gonorrhoeae when exposed to the redox reaction of p-nitro blue chloride tetrazolium (NBT), as formazan precipitation takes place, a shift to lesser fluorescent channels of the histogram population occurs. That effect is labeled NBT-laser beam quenching (NBT-LBQ). A significant difference by Kolmogorov-Smirnov summation curve analysis is found between complement heat-inactivated and its counterpart assessed with normal complement microorganisms. The operation of this NBT-LBQ effect by microbial flow cytometry casts an interesting potential for the evaluation of the outer membrane-complement-interaction of serum sensitive microorganisms
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Indice:
LILAS (Amériques)
Sujet Principal:
Protéines du système du complément
/
Interprétation statistique de données
/
Escherichia coli
/
Cytométrie en flux
/
Fluorescence
/
Neisseria gonorrhoeae
/
Bleu de nitrotétrazolium
langue:
Anglais
Texte intégral:
Arch. med. res
Thème du journal:
Médicament
Année:
1996
Type:
Article
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