Induction of macrophage killing of Leishmania donovani by human CD4+ T cell clones
Arch. med. res
;
27(4): 473-9, 1996. tab, ilus
Article
Dans Anglais
| LILACS
| ID: lil-200349
ABSTRACT
Immunitary in leishmaniasis is mediated by T cells, but protective responses in humans have not been fully defined. In this study, the functional activity of CD4+ T cell clones derived from an immune individual was investigated to identify potentially protective responses. The T cells proliferated and produced interferon-gamma (IFN-gamma) in response to a soluble Leishmania donovani antigen extrac and live amastigotes. There was considerable variation in the anti-leishmanial activity of the T cell clones when they were co-cultured with L. donavani infected monocytes isolated from an HLA-DR,DQ mathced donor. All of the clones which demonstrated antigen specific reactivity by proliferation or cytokineproduction induced some dregee of inhibition of intracellular parasite replication, but only a few of the clones induced pronounced leishmanicidal activity. There was strong correlation between the level of amastigote-induced IFN-gamma secretion and anti-leishmanial activity. This approach anables the identification of potentially protective immune responses in humans at the clonal level, and offers a means for the identification of the relevant antigen(s)
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Indice:
LILAS (Amériques)
Sujet Principal:
Leishmania donovani
/
Agranulocytes
/
Lymphocytes T CD4/
/
Cytokines
/
Cricetinae
/
Leishmaniose cutanée
/
Clonage moléculaire
/
Syndromes lymphoprolifératifs
/
Macrophages
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Arch. med. res
Thème du journal:
Médicament
Année:
1996
Type:
Article
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