Receptor localization of steroid hormones and drugs: discoveries through
Braz. j. med. biol. res
;
31(2): 197-206, feb. 1998.
Article
Dans Anglais
| LILACS
| ID: lil-212570
RESUMO
The history of receptor autoradiography, its development and applications, testify to the utility of this histochemical tecnhique for localizing radiolabeled hormones and drugs at cellular and subcelullar sites of action in intact tissues. Loclization of duffusible compounds has been a challenge that was met through the introduction of the "thaw-mount" and "dry-mount" autoradiographic techniques thirty years ago. With this cellular receptor autoradiography, used alone or combined with other histochemical techniques, sites of specific binding and deposition in vivo and in vitro have been characterized. Numerous discoveries, some reviewed in this article, provided information that led to new concepts and opened new areas of research. As an example, in recent years more than fifty target tissues for vitamin D have been specified, challenging the conventional view about the main biological role of vitamin D. The functions of most of these vitamin D target tissues are unrelated to the regulation of systemic calcium homeostasis, but pertain to the (seasonal) regulation of endo- and exocrine secretion, cell proliferation, reproduction, neural, immune and cardiovascular responses, and adaptation to stress. Receptor autoradiography with cellula resolution has become an indispensable tool in drug research and development, since information can be obtained that is difficult or impossible to gain otherwise.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Autoradiographie
/
Sites de fixation
/
Récepteurs aux stéroïdes
/
Récepteur calcitriol
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1998
Type:
Article
/
Congrès et conférence
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