Production of cyclomaltodextrin glucanotransferase from an alkalophilic Bacillus sp. in rice and corn flakes
Acta cient. venez
;
47(2): 133-7, 1996. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-216824
RESUMO
Cyclomaltodextrin glucanotranferase (CGTase) catalyzes the degradation of starch to form alpha-, beta- and gamma-cyclodextrin. Based on cyclodextrin formation, an alkalophilic Bacillus sp. ATCC 21783 was used for its high CGTase production ability, using corn and rice flakes as substrates. Maximum enzyme production was achieved after 96 hours at pH 9.0, temperature 37 degrees C, and 1 percent(w/v) of either substrate together with the addition of trub. The specific enzyme activity was determined by High Pressure Liquid Chromatography (HPLC) and expressed as using International Units based on total cyclodextrin formation. Optimum conditions for this determination were studied, finding that the best results are obtained at pH 5.0, 7.0 and 9.0, temperature 55 degrees C and 3 hours of incubation in 1 percent (w/v) of rice flakes as starch source
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Indice:
LILAS (Amériques)
Sujet Principal:
Bacillus
/
Glucosyltransferases
langue:
Anglais
Texte intégral:
Acta cient. venez
Thème du journal:
Science
Année:
1996
Type:
Article
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