A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus
Braz. j. med. biol. res
;
32(1): 45-9, Jan. 1999. ilus
Article
Dans Anglais
| LILACS
| ID: lil-226211
RESUMO
We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4 percent) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Amplification de gène
/
Virus de l'hépatite B
/
Réaction de polymérisation en chaîne
/
Génotype
Limites du sujet:
Humains
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1999
Type:
Article
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