Reactivity of anti-thyroid antibodies to thyroglobulin tryptic fragments: comparison of autoimmune and non-autoimmune thyroid diseases
Braz. j. med. biol. res
;
32(4): 449-55, Apr. 1999. ilus, tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-231737
RESUMO
Studies concerning the antigenicity of thyroglobulin fragments allow the characterization of the epitopes but do not consider the role of heavier antigenic fragments that could result in vivo from the action of endoproteases. Here we assess the relative importance of the fragments obtained from thyroglobulin by limited proteolysis with trypsin and compare by immunoblotting their reactivity to serum from patients with autoimmune (Graves' disease and Hashimoto's thyroiditis) and non-autoimmune (subacute thyroiditis) disease. The results showed no difference in frequency of recognition of any peptide by sera from patients with autoimmune thyroiditis. In contrast, sera from patients with subacute thyroiditis reacted more frequently with a peptide of 80 kDa. These results suggest the presence of antibody subpopulations directed at fragments produced in vivo by enzymatic cleavage of thyroglobulin. This fragment and antibodies to it may represent markers for subacute thyroiditis.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Fragments peptidiques
/
Thyroglobuline
/
Thyroïdite subaigüe
/
Thyroïdite auto-immune
/
Maladie de Basedow
/
Anticorps
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1999
Type:
Article
/
descriptif de projet
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