A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus
Braz. j. med. biol. res
;
32(6): 747-52, Jun. 1999. tab, ilus
Article
Dans Anglais
| LILACS
| ID: lil-233707
RESUMO
A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88 per cent), specificity (100 per cent) and agreement (95.31 per cent).
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Virus de la bronchite infectieuse
/
Anticorps antiviraux
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1999
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Brésil
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS