Partial protection of mice against Trypanosoma cruzi after immunizing with the TcY 72 antigenic preparation
Mem. Inst. Oswaldo Cruz
;
94(2): 167-72, Mar.-Apr. 1999. ilus, tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-239012
RESUMO
A 72 kDa Trypanosoma cruzi glycoprotein recognized by the 164C11 monoclonal antibody (IgM isotype) was purified by preparative electrophoresis. The antigenic preparation obtained, named TcY 72, was used to immunize C57Bl/10 mice. The following results were observed after immunization (1) induction of higher titres of IgG than IgM antibodies, as evaluated by indirect immunofluorescence; (2) significant DTH after infection of epimastigotes in mice footpads (3) peak parasitemia in immunized mice was significantly reduced and animals were negative by 13 days post-infection, although the mice still succumb to infection (4) the phenotypic analysis of spleen cell populations showed a decrease in the CD4/CD8 ratio in immunized mice. Taken as a whole, these findings indicate that TcY 72 is immunogenic and potentially important for protective immunity.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma cruzi
/
Anticorps antiprotozoaires
/
Immunisation passive
/
Maladie de Chagas
/
Souris
/
Antigènes de protozoaire
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1999
Type:
Article
Pays d'affiliation:
Brésil
/
France
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