Critical electrolyte concentration of spermatozoal chromatin containing histone H1 variants
Genet. mol. biol
; 22(2): 197-200, jun. 1999. tab
Article
Dans En
| LILACS
| ID: lil-242200
Responsable en Bibliothèque :
BR1.1
ABSTRACT
The critical electrolyte concentrations (CEC) of sperm chromatin from animal species known or suspected to contain histone H1 variants were compared by examining the affinity of their DNA-protein complexes for toluidine blue in the presence of Mg2+. Bullfrog, sea urchin, bee and bumblebee spermatozoa were studied. The CEC for Rana catesbeiana and two sea urchin species were similar to that of histone H5-containing chromatin from chicken erythrocytes, thus confirming the biochemical and structural similarities of these DNA-protein complexes. The CEC for bees and the bumblebee, Bombus atratus, showed no particular phylogenetic relationship. We concluded that the CEC of histone H1-containing sperm cell chromatin is a useful indicator of variability in DNA-protein complexes but is of little phylogenetic value.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Spermatozoïdes
/
Chromatine
/
Histone
/
Électrolytes
Limites du sujet:
Animals
langue:
En
Texte intégral:
Genet. mol. biol
Thème du journal:
GENETICA
Année:
1999
Type:
Article