Integration of Trypanosoma cruzi kDNA minicircle sequence in the host genome may be associated with autoimmune serum factors in Chagas disease patients
Mem. Inst. Oswaldo Cruz
;
94(suppl.1): 249-52, Sept. 1999. ilus
Article
Dans Anglais
| LILACS
| ID: lil-245631
RESUMO
Integration of kDNA sequences within the genome of the host cell shown by PCR amplification with primers to the conserved Trypanosoma cruzi kDNA minicircle sequence was confirmed by Southern hybridization with specific probes. The cells containing the integrated kDNA sequences were then perpetuated as transfected macrophage subclonal lines. The kDNA transfected macrophages expressed membrane antigens that were recognized by antibodies in a panel of sera from ten patients with chronic Chagas disease. These antigens barely expressed in the membrane of uninfected, control macrophage clonal lines were recognized neither by factors in the control, non-chagasic subjects nor in the chagasic sera. This finding suggests the presence of an autoimmune antibody in the chagasic sera that recognizes auto-antigens in the membrane of T. cruzi kDNA transfected macrophage subclonal lines.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma cruzi
/
Auto-immunité
/
Maladie de Chagas
/
ADN kinétoplastique
Type d'étude:
Facteurs de risque
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1999
Type:
Article
/
Congrès et conférence
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