Aspectos que afectan el análisis de contenido de ADN por citometría de flujo / Factors that affect DNA content analysis by flow cytometry
Rev. méd. Chile
;
127(11): 1385-97, nov. 1999. graf
Article
Dans Espagnol
| LILACS
| ID: lil-257999
ABSTRACT
DNA ploidy and cell cycle analysis by flow cytometry is used to obtain additional information about the diagnosis and prognosis of different types of cancer. However, there are several disagreements among authors about the tissue source (fresh-frozen or paraffin embedded), cellular dissociation methods (mechanical, enzymatic or other), use of different dyes, lasers, analysis software with different mathematical models and interpretation of results. A discussion about the different aspects that affect the study of DNA ploidy and cell cycle and a consensus in publications is mandatory. A strict control of analysis processes and data interpretation is also necessary
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
ADN
/
Cytométrie en flux
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Espagnol
Texte intégral:
Rev. méd. Chile
Thème du journal:
Médicament
Année:
1999
Type:
Article
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