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Evaluation of an enzyme immunoassay for hepatitis C virus antibody detection using a recombinant protein derived from the core region of hepatitis C virus genome
Lopes, E. P. A; Granato, C. H; Lanzoni, V; Granero, L; Paranhos-Baccala, G; Tomiyama, H; Silva, A. E. B; Ferraz, M. L.
  • Lopes, E. P. A; Universidade Federal de Sao Paulo. Escola Paulista de Medicina. Sao Paulo. BR
  • Granato, C. H; Universidade Federal de Sao Paulo. Escola Paulista de Medicina. Sao Paulo. BR
  • Lanzoni, V; Universidade Federal de Sao Paulo. Escola Paulista de Medicina. Sao Paulo. BR
  • Granero, L; Universidade Federal de Sao Paulo. Escola Paulista de Medicina. Sao Paulo. BR
  • Paranhos-Baccala, G; Instituto BioMérieux. Lyon. FR
  • Tomiyama, H; Universidade Federal de Sao Paulo. Escola Paulista de Medicina. Sao Paulo. BR
  • Silva, A. E. B; Universidade Federal de Sao Paulo. Escola Paulista de Medicina. Sao Paulo. BR
  • Ferraz, M. L; Universidade Federal de Sao Paulo. Escola Paulista de Medicina. Sao Paulo. BR
Mem. Inst. Oswaldo Cruz ; 95(5): 717-20, Sept.-Oct. 2000. tab, graf
Article Dans Anglais | LILACS | ID: lil-267901
ABSTRACT
This study was undertaken to evaluate an enzyme immunoassay (EIA) for hepatitis C virus antibody detection (anti-HCV), using just one antigen. Anti-HCV EIA was designed to detect anti-HCV IgG using on the solid-phase a recombinant C22 antigen localized at the N-terminal end of the core region of HCV genome, produced by BioMérieux. The serum samples diluted in phosphate buffer saline were added to wells coated with the C22, and incubated. After washings, the wells were loaded with conjugated anti-IgG, and read in a microtiter plate reader (492 nm). Serum samples of 145 patients were divided in two groups a control group of 39 patients with non-C hepatitis (10 acute hepatitis A, 10 acute hepatitis B, 9 chronic hepatitis B, and 10 autoimmune hepatitis) and a study group consisting of 106 patients with chronic HCV hepatitis. In the study group all patients had anti-HCV detected by a commercially available EIA (Abbott(r)), specific for HCV structural and nonstructural polypeptides, alanine aminotransferase elevation or positive serum HCV-RNA detected by nested-PCR. They also had a liver biopsy compatible with chronic hepatitis. The test was positive in 101 of the 106 (95 percent) sera from patients in the study group and negative in 38 of the 39 (97 percent) sera from those in the control group, showing an accuracy of 96 percent. According to these results, our EIA could be used to detect anti-HCV in the serum of patients infected with hepatitis C virus.
Sujets)
Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Protéines recombinantes / Protéines du core viral / Génome viral / Hepacivirus / Anticorps de l'hépatite C Type d'étude: Etude diagnostique Limites du sujet: Adolescent / Adulte / Femelle / Humains / Mâle langue: Anglais Texte intégral: Mem. Inst. Oswaldo Cruz Thème du journal: Médecine tropicale / Parasitologie Année: 2000 Type: Article / descriptif de projet Pays d'affiliation: Brésil / France Institution/Pays d'affiliation: Instituto BioMérieux/FR / Universidade Federal de Sao Paulo/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Protéines recombinantes / Protéines du core viral / Génome viral / Hepacivirus / Anticorps de l'hépatite C Type d'étude: Etude diagnostique Limites du sujet: Adolescent / Adulte / Femelle / Humains / Mâle langue: Anglais Texte intégral: Mem. Inst. Oswaldo Cruz Thème du journal: Médecine tropicale / Parasitologie Année: 2000 Type: Article / descriptif de projet Pays d'affiliation: Brésil / France Institution/Pays d'affiliation: Instituto BioMérieux/FR / Universidade Federal de Sao Paulo/BR