Characterization and optimization of bovine Echinococcus granulosus cyst fluid to be used in immunodiagnosis of hydatid disease by ELISA
Rev. Inst. Med. Trop. Säo Paulo
;
42(5): 255-62, Sept.-Oct. 2000. tab, ilus
Article
Dans Anglais
| LILACS
| ID: lil-270226
RESUMO
The aim of this work was to assess the influence in the diagnostic value for human hydatid disease of the composition of bovine hydatid cyst fluid (BHCF) obtained from fertile (FC) and non-fertile cysts (NFC). Eight batches from FC and 5 from NFC were prepared and analysed with respect to chemical composition total protein, host-derived protein, carbohydrate and lipid contents. No differences were observed in the first two parameters but carbohydrate and lipid contents were shown to be higher in batches from FC than in those from NFC. Bands of 38 and 116 kD in SDS-PAGE profiles were observed to be present in BHCF from FC only. Two pools were prepared from BHCF batches obtained from FC (PFC) and NFC (PNFC), respectively. Antigen recognition patterns were analysed by immunoblot. Physicochemical conditions for adsorption of antigens to the polystyrene surface (ELISA plates) were optimized. The diagnostic value of both types of BHCF as well as the diagnostic relevance of oxidation of their carbohydrate moieties with periodate were assessed by ELISA using 42 serum samples from hydatid patients, 41 from patients with other disorders, and 15 from healthy donors. Reactivity of all sera against native antigen were tested with and without free phosphorylcholine. The best diagnostic efficiency was observed using BHCF from periodate-treated PFC using glycine buffer with strong ionic strength to coat ELISA plates
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Anticorps antihelminthe
/
Liquide kystique
/
Échinococcose
/
Echinococcus
/
Antigènes d'helminthe
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Rev. Inst. Med. Trop. Säo Paulo
Thème du journal:
Médecine tropicale
Année:
2000
Type:
Article
Pays d'affiliation:
Uruguay
Institution/Pays d'affiliation:
Universidad de la República/UY
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS