Polymerase chain reaction for the laboratory diagnosis of aseptic meningitis and encephalitis
Arq. neuropsiquiatr
;
58(3B): 836-42, Sept. 2000.
Article
Dans Anglais
| LILACS
| ID: lil-273108
ABSTRACT
A protocol for testing cerebrospinal fluid specimens using a range of PCR assays for the diagnosis of central nervous system infection was developed and used to test prospectively 383 specimens. PCR assays were used for the detection of adenovirus, Borrelia burgdorferi, enteroviruses, Epstein Barr virus, cytomegalovirus, herpes simplex virus, human herpes virus type 6, JC virus, Leptospira interrogans, Listeria monocytogenes, lymphocytic choriomeningitis virus, measles virus, mumps virus, Mycobacterium sp., Mycoplasma pneumoniae, Toxoplasma gondii and varicella zoster virus. Of the 383 specimens tested in this study, 46 (12.0 percent) were found to be positive. The microorganisms detected were CMV, enterovirus, Epstein Barr virus, herpes simplex virus, human herpes virus type 6, JC virus, L. monocytogenes, Mycobacterium genus, Toxoplasma gondii and varicella zoster virus. The introduction of the PCR protocol described has improved the diagnosis of a range of central nervous system infections in our laboratory. We believe however that further evaluation of these assays in immunocompromised patients is necessary to better determine the predictive value of positive PCR results in these patient groups
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Réaction de polymérisation en chaîne
/
Encéphalite
/
Méningite aseptique
Type d'étude:
Etude diagnostique
/
Etude d'étiologie
/
Guide de pratique
/
Étude pronostique
Limites du sujet:
Adolescent
/
Adulte
/
Enfant
/
Enfant d'âge préscolaire
/
Humains
langue:
Anglais
Texte intégral:
Arq. neuropsiquiatr
Thème du journal:
Neurologie
/
Psychiatrie
Année:
2000
Type:
Article
Pays d'affiliation:
Brésil
/
Royaume-Uni
Institution/Pays d'affiliation:
Public Health Laboratory/GB
/
Universidade Federal do Rio Grande do Sul/BR
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