Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification
Mem. Inst. Oswaldo Cruz
;
96(4): 535-544, May 2001. ilus, tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-285549
ABSTRACT
Biomphalaria glabrata, B. tenagophila and B. straminea are intermediate hosts of Schistosoma mansoni, in Brazil. The latter is of epidemiological importance in the northwest of Brazil and, due to morphological similarities, has been grouped with B. intermedia and B. kuhniana in a complex named B. straminea. In the current work, we have standardized the simple sequence repeat anchored polymerase chain reaction (SSR-PCR) technique, using the primers (CA)8RY and K7, to study the genetic variability of these species. The similarity level was calculated using the Dice coefficient and genetic distance using the Nei and Li coefficient. The trees were obtained by the UPGMA and neighbor-joining methods. We have observed that the most related individuals belong to the same species and locality and that individuals from different localities, but of the same species, present clear heterogeneity. The trees generated using both methods showed similar topologies. The SSR-PCR technique was shown to be very efficient in intrapopulational and intraspecific studies of the B. straminea complex snails
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Variation génétique
/
Biomphalaria
/
Réaction de polymérisation en chaîne
/
Répétitions minisatellites
/
Vecteurs insectes
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2001
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Fiocruz/BR
/
Instituto Ludwig de Pesquisas sobre Câncer/BR
/
UFMG/BR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS