Screening of bacterial strains for pectinolytic activity: characterization of the polygalacturonase produced by "Bacillus" sp
Rev. microbiol
;
30(4): 299-303, out.-dez. 1999. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-286783
RESUMO
One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use of citrus pectin as the sole carbon source. 102 were positive for pectinase depolymerization in assay plates as evidenced by clear hydrolization halos. Among them, 30(per cent) presented considerable pectinolytic activity. The cultivation of these strains by submerged and semi-solid fermentation for polygalacturose production indicated that five strains of "Bacillus" sp. produced high quantities of the enzyme. The physico-chemical characteristics, such as optimum pH of 6.0-7.0, optimum temperatures between 45ºC and 55ºC, stability at temperatures above 40ºC and in neutral and alkaline pH, were determined
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Polygalacturonase
/
Bacillus
Type d'étude:
Etude diagnostique
/
Étude de dépistage
langue:
Anglais
Texte intégral:
Rev. microbiol
Thème du journal:
Microbiologie
Année:
1999
Type:
Article
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