In situ hybridization of somatolactin transcripts in the pituitary glands from acclimatized carp (Cyprinus carpio)
Biol. Res
; 34(1): 7-13, 2001. ilus
Article
de En
| LILACS
| ID: lil-288328
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BR1.1
RESUMO
We isolated and cloned a carp somatolactin SL DNA fragment, of which 78 per cent of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter-acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the regulation of the somatolactin detected with the probe used at the transcriptional level in pituitary glands.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Hypophyse
/
Hormones hypophysaires
/
Transcription génétique
/
Hybridation in situ
/
Acclimatation
Limites du sujet:
Animals
langue:
En
Texte intégral:
Biol. Res
Thème du journal:
BIOLOGIA
Année:
2001
Type:
Article