Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
Braz. j. med. biol. res
;
34(9): 1175-1183, Sept. 2001. ilus, graf
Article
Dans Anglais
| LILACS
| ID: lil-290404
ABSTRACT
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Angiotensine-II
/
Récepteurs aux angiotensines
/
Cellules CHO
/
Endocytose
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2001
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal de Säo Paulo/BR
/
Universidade de Säo Paulo/BR
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