Time course of virus-like particles (VLPS) double-stranded RNA accumulation in toxigenic and non-toxigenic strains of Aspergillus flavus

Silva, Valéria N; Durigon, Edison Luiz; Pires, Maria de Fátima Costa; Lourenço, Alexandre; Faria, Maria Jacinta de; Corrêa, Benedito

Silva, Valéria N; Durigon, Edison Luiz; Pires, Maria de Fátima Costa; Lourenço, Alexandre; Faria, Maria Jacinta de; Corrêa, Benedito.

Silva, Valéria N; Universidade de Säo Paulo. Instituo de Ciências Biomédicas.
Durigon, Edison Luiz; Universidade de Säo Paulo. Instituto de Ciências Biomédicas.
Pires, Maria de Fátima Costa; Instituto Adolfo Luz. Divisäo de Virologia.
Lourenço, Alexandre; Universidade de Säo Paulo. Instituto de Ciências Biomédicas.
Faria, Maria Jacinta de; Universidade de Säo Paulo. Instituto de Ciências Biomédicas.
Corrêa, Benedito; Universidade de Säo Paulo. Instituo de Ciências Biomédicas.

Braz. j. microbiol ; 32(1): 56-60, Jan.-Mar. 2001. ilus, tab, graf

Article Dans Anglais | LILACS | ID: lil-297668

ABSTRACT

ABSTRACT

Two strains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRRL 5940, were studied over a period of 44 days, in order to detect the presence of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids electrophoresis. Only the toxigenic strain contained VLPs, presenting three-segmented dsRNA. An increase in VLPs number was observed during the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication.

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Aspergillus flavus / Virus à ARN / Techniques in vitro / ARN langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2001 Type: Article Pays d'affiliation: Brésil

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