Identification of a differentially expressed mRNA in axenic Leishmania panamensis amastigotes
Mem. Inst. Oswaldo Cruz
;
96(6): 835-838, Aug. 2001. ilus
Article
Dans Anglais
| LILACS
| ID: lil-298594
RESUMO
Differential display technique was applied in order to identify transcripts which are present in axenic amastigotes but not in promastigotes of the Leishmania panamensis parasites. One of them was cloned and the sequence reveals an open reading frame of 364 amino acids (aprox. 40 kDa). The deduced protein is homologous to the serine/threonine protein kinases and specially to the mitogen activates protein kinases from eukaryotic species. Southern blot analysis suggest that this transcript, named lpmkh, is present in the genome of the parasite as a single copy gene. These results could imply that lpmkh could be involved in the differentiation process or the preservation of amastigotes in axenic conditions
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
ARN messager
/
Leishmania
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
Pays comme sujet:
Amérique centrale
/
Panama
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2001
Type:
Article
Pays d'affiliation:
Colombie
Institution/Pays d'affiliation:
Universidad Nacional de Colombia/CO
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