Your browser doesn't support javascript.
loading
p53 gene analysis in childhood B non - Hodgkin's lymphoma
Klumb, Claudete Esteves Nogueira Pinto; Resende, Lídia Maria Magalhäes de; Tajara, Eloísa Helena; Bertelli, Erika Cristina Pavarino; Rumjanek, Vivian Mary; Maia, Raquel Ciuvalschi.
  • Klumb, Claudete Esteves Nogueira Pinto; National Cancer Institute. Hospital do Câncer. Hematology Service. Laboratory of Cellular and Molecular Hematology. Rio de Janeiro. BR
  • Resende, Lídia Maria Magalhäes de; National Cancer Institute. Hospital do Câncer. Pathology Service. Rio de Janeiro. BR
  • Tajara, Eloísa Helena; Universidade Estadual Paulista. Instituto de Biociências Letras e Exatas. Department of Biology. Säo José do Rio Preto. BR
  • Bertelli, Erika Cristina Pavarino; Faculty of Medicine. Department of Molecular Biology. Säo José do Rio Preto. BR
  • Rumjanek, Vivian Mary; Universidade Federal do Rio de Janeiro. Department of Biochemistry. Laboratory of Tumoral Immunology. Rio de Janeiro. BR
  • Maia, Raquel Ciuvalschi; National Cancer Institute. Hospital do Câncer. Hematology Service. Laboratory of Cellular and Molecular Hematology. Rio de Janeiro. BR
São Paulo med. j ; 119(6): 212-215, Nov. 2001. ilus, tab
Article Dans Anglais | LILACS | ID: lil-299791
ABSTRACT
CONTEXT Mutations or deletions in the tumor-suppressor gene p53 are among the commonest genetic changes found in human neoplasms including breast, lung and bowel cancers. In hematological malignancies, p53 is most often mutated in Burkitt's lymphoma, with p53 mutations present in 30 to 40 percent of tumor samples and in 70 percent of cell lines.

OBJECTIVE:

To analyze the p53 gene alterations in child patients with B non-Hodgkin's lymphoma.

DESIGN:

Descriptive study.

SETTING:

Tertiary oncology care center.

PARTICIPANTS:

The study investigated 12 patients with childhood B non-Hodgkin's lymphoma (Burkitt's lymphoma). Screening for p53 mutations was done by polymerase chain reaction - single strand conformational polymorphism (PCR-SSCP) analysis of exon 5 to 8/9 of the gene.

RESULTS:

Abnormal polymerase chain reaction - single strand conformational polymorphism migration pattern was observed in 4 patients (33.3 percent), one on exon 6 and three on exon 7. Positive cases included 2 patients who died from disease.

CONCLUSION:

These preliminary results suggest that p53 mutations are quite frequent in children with Burkitt's lymphoma and may play a role in lymphoma genesis or disease progression
Sujets)
Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Gènes p53 / Lymphome de Burkitt / Mutation Limites du sujet: Enfant / Enfant d'âge préscolaire / Femelle / Humains / Mâle langue: Anglais Texte intégral: São Paulo med. j Thème du journal: Cirurgia Geral / Ciˆncia / Ginecologia / Médicament / Medicina Interna / Obstetr¡cia / Pediatria / Sa£de Mental / Sa£de P£blica Année: 2001 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Faculty of Medicine/BR / National Cancer Institute/BR / Universidade Estadual Paulista/BR / Universidade Federal do Rio de Janeiro/BR

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Gènes p53 / Lymphome de Burkitt / Mutation Limites du sujet: Enfant / Enfant d'âge préscolaire / Femelle / Humains / Mâle langue: Anglais Texte intégral: São Paulo med. j Thème du journal: Cirurgia Geral / Ciˆncia / Ginecologia / Médicament / Medicina Interna / Obstetr¡cia / Pediatria / Sa£de Mental / Sa£de P£blica Année: 2001 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Faculty of Medicine/BR / National Cancer Institute/BR / Universidade Estadual Paulista/BR / Universidade Federal do Rio de Janeiro/BR