p53 gene analysis in childhood B non - Hodgkin's lymphoma
São Paulo med. j
;
119(6): 212-215, Nov. 2001. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-299791
ABSTRACT
CONTEXT Mutations or deletions in the tumor-suppressor gene p53 are among the commonest genetic changes found in human neoplasms including breast, lung and bowel cancers. In hematological malignancies, p53 is most often mutated in Burkitt's lymphoma, with p53 mutations present in 30 to 40 percent of tumor samples and in 70 percent of cell lines. OBJECTIVE:
To analyze the p53 gene alterations in child patients with B non-Hodgkin's lymphoma.DESIGN:
Descriptive study.SETTING:
Tertiary oncology care center.PARTICIPANTS:
The study investigated 12 patients with childhood B non-Hodgkin's lymphoma (Burkitt's lymphoma). Screening for p53 mutations was done by polymerase chain reaction - single strand conformational polymorphism (PCR-SSCP) analysis of exon 5 to 8/9 of the gene.RESULTS:
Abnormal polymerase chain reaction - single strand conformational polymorphism migration pattern was observed in 4 patients (33.3 percent), one on exon 6 and three on exon 7. Positive cases included 2 patients who died from disease.CONCLUSION:
These preliminary results suggest that p53 mutations are quite frequent in children with Burkitt's lymphoma and may play a role in lymphoma genesis or disease progression
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Gènes p53
/
Lymphome de Burkitt
/
Mutation
Limites du sujet:
Enfant
/
Enfant d'âge préscolaire
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
São Paulo med. j
Thème du journal:
Cirurgia Geral
/
Cincia
/
Ginecologia
/
Médicament
/
Medicina Interna
/
Obstetr¡cia
/
Pediatria
/
Sa£de Mental
/
Sa£de P£blica
Année:
2001
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Faculty of Medicine/BR
/
National Cancer Institute/BR
/
Universidade Estadual Paulista/BR
/
Universidade Federal do Rio de Janeiro/BR
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