Molecular identification of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea in Londrina, Parana, Brazil
Braz. j. microbiol
;
31(4): 275-280, oct.-dec. 2000. ilus, tab
Article
Dans Anglais, Portugais
| LILACS
| ID: lil-299824
RESUMO
In this work, the prevalence of enteropathogenic Escherichia coli (EPEC) in children in Londrina-PR, Brazil, was evaluated by means of digoxigenin-labelled DNA probes which identify the plasmid responsible for EPEC adherence factor (EAF), and virulence genes for EPEC as bundle-forming pilus (bfp) and E. coli attaching-effacing factor (eae). In addition, the isolated strains were serotyped and tested for adherence to HEp-2 cells. From 102 children with diarrhoea, 19 strains hybridized with at least one probe, and eleven of them were identified as typical EPEC because they hybridized with the three probes used, showed a localized adherence (LA) pattern, and presented no genes for enterotoxins (ST and LT) or invasion as detected by PCR. Six of the typical EPEC strains belonged to the classical serotype O119H6 43(per cent); in four strains O antigens could not be determined using antisera against O1 to O173, they were all ONTH7 29(per cent); one strain belonged to O111H6. Three strains were classified as atypical EPEC O26H-, O111H9 and O119HNT. Strains O26H- and O111H9 hybridized with the eae probe only and showed localized adherence like (LAL) pattern; strain O119HNT hybridized with the bfp and eae probes, and showed a localized adherence/diffuse adherence (LA/DA) pattern after 6 h. A DA pattern was observed in two strains isolated from children with diarrhoea (ONTH11 and O142H34), which hybridized with the eae probe. From 46 controls, five strains hybridized with one or two probes, but none hybridized with all probes or presented the LA pattern. Three strains with the DA pattern hybridized with the eae probe. No EPEC strain belonging to classical EPEC serotypes was isolated from faeces of control children.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Diarrhée du nourrisson
/
Escherichia coli
/
Infections à Escherichia coli
Type d'étude:
Etude diagnostique
/
Étude pronostique
/
Facteurs de risque
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
/
Portugais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2000
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Instituto Adolfo Lutz/BR
/
Universidade Estadual de Londrina/BR
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