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In vivo and in vitro effect of imipramine and fluoxetine on Na+, K+-ATPase activity in synaptic plasma membranes from the cerebral cortex of rats
Zanatta, L. M; Nascimento, F. C; Barros, S. V. T; Silva, G. R. R. S; Zugno, A. I; Netto, C. A; Wyse, A. T. S.
  • Zanatta, L. M; Fundaçäo Universidade do Rio Grande. Departamento de Medicina Interna. Rio Grande. BR
  • Nascimento, F. C; Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Bioquímica. Porto Alegre. BR
  • Barros, S. V. T; Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Bioquímica. Porto Alegre. BR
  • Silva, G. R. R. S; Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Bioquímica. Porto Alegre. BR
  • Zugno, A. I; Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Bioquímica. Porto Alegre. BR
  • Netto, C. A; Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Bioquímica. Porto Alegre. BR
  • Wyse, A. T. S; Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Bioquímica. Porto Alegre. BR
Braz. j. med. biol. res ; 34(10): 1265-1269, Oct. 2001. graf
Article Dans Anglais | LILACS | ID: lil-299841
RESUMO
The effects of in vivo chronic treatment and in vitro addition of imipramine, a tricyclic antidepressant, or fluoxetine, a selective serotonin reuptake inhibitor, on the cortical membrane-bound Na+,K+-ATPase activity were studied. Adult Wistar rats received daily intraperitoneal injections of 10 mg/kg of imipramine or fluoxetine for 14 days. Twelve hours after the last injection rats were decapitated and synaptic plasma membranes (SPM) from cerebral cortex were prepared to determine Na+,K+-ATPase activity. There was a significant decrease (10 percent) in enzyme activity after imipramine but fluoxetine treatment caused a significant increase (27 percent) in Na+,K+-ATPase activity compared to control (P<0.05, ANOVA; N = 7 for each group). When assayed in vitro, the addition of both drugs to SPM of naive rats caused a dose-dependent decrease in enzyme activity, with the maximal inhibition (60-80 percent) occurring at 0.5 mM. We suggest that a) imipramine might decrease Na+,K+-ATPase activity by altering membrane fluidity, as previously proposed, and b) stimulation of this enzyme might contribute to the therapeutic efficacy of fluoxetine, since brain Na+,K+-ATPase activity is decreased in bipolar patients
Sujets)
Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Membranes synaptiques / Cortex cérébral / Fluoxétine / Sodium-Potassium-Exchanging ATPase / Imipramine / Antidépresseurs Limites du sujet: Animaux langue: Anglais Texte intégral: Braz. j. med. biol. res Thème du journal: Biologie / Médicament Année: 2001 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Fundaçäo Universidade do Rio Grande/BR / Universidade Federal do Rio Grande do Sul/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Membranes synaptiques / Cortex cérébral / Fluoxétine / Sodium-Potassium-Exchanging ATPase / Imipramine / Antidépresseurs Limites du sujet: Animaux langue: Anglais Texte intégral: Braz. j. med. biol. res Thème du journal: Biologie / Médicament Année: 2001 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Fundaçäo Universidade do Rio Grande/BR / Universidade Federal do Rio Grande do Sul/BR