In vitro cytotoxicity of the LDE-daunorubicin complex in acute myelogenous leukemia blast cells
Braz. j. med. biol. res
;
34(10): 1257-1263, Oct. 2001. ilus, graf
Article
Dans Anglais
| LILACS
| ID: lil-299847
RESUMO
Acute myelogenous leukemia (AML) blast cells show high-affinity degradation of low-density lipoprotein (LDL), suggesting an increased expression of cellular LDL receptors. LDE is a lipid microemulsion easily synthesized in vitro which is known to mimic the metabolic pathway of LDL. We used LDE as a carrier for daunorubicin and assayed the cytotoxicity of the complex using AML blast cells since RT-PCR analysis showed that AML cells express LDL receptor mRNA. The LDEdaunorubicin complex killed 46.7 percent of blast cells and 20.2 percent of normal bone marrow cells (P<0.001; Student t-test). Moreover, this complex destroyed AML blast cells as efficiently as free daunorubicin. Thus, LDE might be a suitable carrier of chemotherapeutic agents targeting these drugs to neoplastic cells and protecting normal tissues
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Cellules souches tumorales
/
Leucémie aigüe myéloïde
/
Daunorubicine
/
Lipoprotéines LDL
/
Antibiotiques antinéoplasiques
Limites du sujet:
Adolescent
/
Adulte
/
Enfant
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2001
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade de Säo Paulo/BR
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