Infiltrating ductal mammary carcinoma: lectin Histochemistry study
An. Fac. Med. Univ. Fed. Pernamb
;
46(1): 32-35, 2001. ilus
Article
Dans Anglais
| LILACS
| ID: lil-299915
RESUMO
Parkia pendula seed lectin (PpeL) was evaluated as histochemical marker for human infiltrating duct carcinoma. Concanavalin A (Con A) was used as pattern of tissue lectin binding. Tissues were treated with 0,1 por cento trypsin solution and 0,3 por cento methanol-H2. Optic microscopy waas used to evaluate the lectin-binding pattern to tissue. Methyl-a-D-mannoside, at 300nM, was used to inhibit the lectin binding to mammary tissues. The inhibition assay reinforced that Ppel conjugated to peroxidase bound to glucose and/or mannose residues in the membrane surface glycoconjugates. Distinct binding patterns of Ppel, associated to different mammary epithelium dedifferentiation, were comparable to those obtained with Con A. These findings indicate a potencial use of this lectin as histochemical marker in investigations of breast neoplasic alterations
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Indice:
LILAS (Amériques)
Sujet Principal:
Région mammaire
/
Carcinome canalaire du sein
/
Lectines
Limites du sujet:
Adulte
/
Humains
langue:
Anglais
Texte intégral:
An. Fac. Med. Univ. Fed. Pernamb
Année:
2001
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
CNPq/BR
/
UFPE/BR
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