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Biological and genetic characteristics of uropathogenic Escherichia coli strains
Silveira, Wanderley Dias da; Benetti, Fabiane; Lancellotti, Marcelo; Ferreira, Alessandra; Solferini, Vera Nisaka; Brocchi, Marcelo.
  • Silveira, Wanderley Dias da; Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Microbiologia e Imunologia. Campinas. BR
  • Benetti, Fabiane; Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Microbiologia e Imunologia. Campinas. BR
  • Lancellotti, Marcelo; Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Microbiologia e Imunologia. Campinas. BR
  • Ferreira, Alessandra; Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Microbiologia e Imunologia. Campinas. BR
  • Solferini, Vera Nisaka; Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética e Evoluçäo. Campinas. BR
  • Brocchi, Marcelo; Universidade de Säo Paulo. Faculdade de Medicina de Ribeiräo Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos. Ribeiräo Preto. BR
Rev. Inst. Med. Trop. Säo Paulo ; 43(6): 303-310, Nov.-Dec. 2001. ilus, graf, tab
Article Dans Anglais | LILACS | ID: lil-303039
RESUMO
The aim of the present study was to determine biological characteristics such as expression of fimbriae, Congo red binding, production of hemolysin and aerobactin, adhesion to HeLa and uroepithelial cells and invasion of HeLa cells by Escherichia coli isolates obtained from patients showing clinical signs of urinary tract infection (UTI). Also, the presence of genes (apa, afa, spa) for fimbria expression and cytotoxic necrotizing factors (CNF1, CNF2) was assayed using specific primers in PCR. The data obtained were compared with the clonal relationships obtained by analysis of multilocus enzyme electrophoresis (MLEE), restriction fragment length polymorphism (RFLP) of the rDNA (ribotyping) and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). All isolates but one presented a combination of at least two of the characteristics studied, a fact suggesting the presence of pathogenicity islands (PAIs). Diffuse adherence type to HeLa cells was observed to occur in most of the strains, but adhesion to uroepithelial cells seems to be a more reliable test to verify pathogenicity. Although four strains seemed to be able to invade HeLa cells when assayed by light microscopy, electron microscopy studies demonstrated that these strains were not invasive. MLEE, RFLP and ERIC-PCR were able to group the isolates differently into main clusters that were not correlated with the presence of pathogenic traits
Sujets)
Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Infections urinaires / Escherichia coli / Infections à Escherichia coli Limites du sujet: Humains langue: Anglais Texte intégral: Rev. Inst. Med. Trop. Säo Paulo Thème du journal: Médecine tropicale Année: 2001 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Estadual de Campinas/BR / Universidade de Säo Paulo/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Infections urinaires / Escherichia coli / Infections à Escherichia coli Limites du sujet: Humains langue: Anglais Texte intégral: Rev. Inst. Med. Trop. Säo Paulo Thème du journal: Médecine tropicale Année: 2001 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Estadual de Campinas/BR / Universidade de Säo Paulo/BR