Bone marrow fibroblasts in patients with advanced lung cancer
Braz. j. med. biol. res
;
34(11): 1457-1463, Nov. 2001. tab
Article
Dans Anglais
| LILACS
| ID: lil-303323
ABSTRACT
In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of pure fibroblast cultures were measured with an ELISA kit and RIA kit, respectively. Only fibroblasts from 6/13 (46 percent) LCP confluent primary cultures had the capacity to proliferate following four subcultures (NC = 100 percent). Levels of spontaneously released IL-1ß were below 10 pg/ml in the CM of LCP, while NC had a mean value of 1,217 + or - 74 pg/ml. In contrast, levels of PGE2 in these CM of LCP were higher (77.5 + or - 23.6 pg/ml) compared to NC (18.5 + or - 0.9 pg/ml). In conclusion, bone marrow fibroblasts from LCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1ß and PGE2 production
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Cellules de la moelle osseuse
/
Carcinome pulmonaire non à petites cellules
/
Fibroblastes
/
Tumeurs du poumon
Type d'étude:
Étude observationnelle
/
Facteurs de risque
Limites du sujet:
Adulte
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
2001
Type:
Article
/
Congrès et conférence
Pays d'affiliation:
Argentine
Institution/Pays d'affiliation:
Hospital Británico/AR
/
I. Iriarte Hospital/AR
/
Instituto de Biología y Medicina Experimental/AR
/
Research Member of the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET)/AR
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