Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes
J. venom. anim. toxins
;
7(2): 276-301, 2001. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-303718
ABSTRACT
The aim of this study was to compare individual venom samples of Crotalus durissus terrificus recently captured in the wild to evaluate possible differences in venom protein composition. Protein levels were quantified by biochemical method (Biuret) and then submitted to electrophoresis. Electrophoresis studies of native protein were performed in vertical slabs of polyacrylamide gel (PAGE), in an alkaline discontinuous buffer system, with a concentration of 10 per cent in the separation gel. SDS-PAGE was performed in PhastGel (8-25). Both gels were stained with Coomassie Blue. Gels were analyzed using the VDS-Pharmacia device. Our results indicate that all analyzed venom samples showed different protein composition, although common protein fractions were detected in some individual samples. Differences were observed between the different individual venom samples and so in the same specimen in relation to the time of collection, for both techniques used. Diet did not influence the variability of venom composition. There is a significant difference in native venom protein composition of males and females.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Brésil
/
Crotalus
/
Venins de crotalidé
/
Électrophorèse sur gel de polyacrylamide
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
J. venom. anim. toxins
Thème du journal:
Toxicologie
Année:
2001
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Estadual Paulista/BR
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