Vancomycin monitoring in term newborns: comparison of peak and trough serum concentrations determined by high performance liquid chromatography and fluorescence polarization immunoassay
Rev. Hosp. Clin. Fac. Med. Univ. Säo Paulo
;
56(5): 149-152, Sept.-Oct. 2001. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-305076
RESUMO
INTRODUCTION:
Peak and trough serum concentrations of vancomycin were determined in term newborn infants with confirmed or suspected Staphylococcus sp sepsis by high performance liquid chromatography and flourescence polarization immunoassay.OBJECTIVE:
To statistically compare the results of the high performance liquid chromatography and flourescence polarization immunoassay techniques for measuring serum vancomycin concentrations.METHODS:
Eighteen peak and 20 trough serum samples were assayed for vancomycin concentrations using high performance liquid chromatography and flourescence polarization immunoassay from October 1995 to October 1997.RESULTS:
The linear correlation coefficients for high performance liquid chromatography and flourescence polarization immunoassay were 0.27 (peak, P = 0.110) and 0.26 (trough, P = 0.1045) respectively, which were not statistically significant.CONCLUSION:
There was wide variation in serum vancomycin concentrations determined by high performance liquid chromatography as compared with those determined by flourescence polarization immunoassay. There was no recognizable pattern in the variability; in an apparently random fashion, the high performance liquid chromatography measurement was sometimes substantially higher than the flourescence polarization immunoassay measurement, and at other times it was substantially lower
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Vancomycine
/
Dosage immunologique par polarisation de fluorescence
/
Chromatographie en phase liquide à haute performance
Limites du sujet:
Humains
/
Nouveau-né
langue:
Anglais
Texte intégral:
Rev. Hosp. Clin. Fac. Med. Univ. Säo Paulo
Thème du journal:
Médicament
Année:
2001
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
University of Säo Paulo/BR
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