A heminested polymerase chain reaction for the detection of Brazilian rabies isolates from vampire bats and herbivores
Mem. Inst. Oswaldo Cruz
;
97(1): 109-111, Jan. 2002. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-306075
ABSTRACT
A heminested-PCR (hn-PCR) using primers to the nucleoprotein-coding gene in a nested set was evaluated in the detection of Brazilian strains of rabies virus (RV). A representative number of RV nucleoprotein sequences belonging to genotype 1 were aligned. Based on such alignment, primers were directed to highly conserved regions. All 42 clinical samples positive by both fluorescent antibody and mouse inoculation tests were also positive by the hn-PCR. Brain tissue that had been left to decompose, obtained from an experimentally inoculated mouse was tested by hn-PCR and yielded positive results. In conclusion, primers designed here were capable of amplifying Brazilian RV isolates obtained from a rural epidemiological cycle
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Rage (maladie)
/
Virus de la rage
/
Réaction de polymérisation en chaîne
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2002
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Santo Amaro/BR
/
Universidade de Säo Paulo/BR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS