Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat
Rev. Inst. Med. Trop. Säo Paulo
;
43(5): 247-250, Sept.-Oct. 2001. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-307996
RESUMO
The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10-7, 10-8 or 10-9 CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1 percent) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10-9 CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Produits de basse-cour
/
Salmonella
/
ADN bactérien
/
Poulets
Type d'étude:
Etude diagnostique
/
Guide de pratique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Rev. Inst. Med. Trop. Säo Paulo
Thème du journal:
Médecine tropicale
Année:
2001
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Federal University of Rio Grande do Sul/BR
/
Federal University of Santa Maria/BR
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