Anionic sites, fucose residues and Class I human leukocyte antigen fate during interaction of Toxoplasma gondii with endothelial cells
Mem. Inst. Oswaldo Cruz
;
97(4): 517-522, June 2002. ilus
Article
Dans Anglais
| LILACS
| ID: lil-314526
ABSTRACT
Toxoplasma gondii invades and proliferates in human umbilical vein endothelial cells where it resides in a parasitophorous vacuole. In order to analyze which components of the endothelial cell plasma membrane are internalized and become part of the parasitophorous vacuole membrane, the culture of endothelial cells was labeled with cationized ferritin or UEA I lectin or anti Class I human leukocytte antigen (HLA) before or after infection with T. gondii. The results showed no cationized ferritin and UEA I lectin in any parasitophorous vacuole membrane, however, the Class I HLA molecule labeling was observed in some endocytic vacuoles containing parasite until 1 h of interaction with T. gondii. After 24 h parasite-host cell interaction, the labeling was absent on the vacuolar membrane, but presents only in small vesicles near parasitophorous vacuole. These results suggest the anionic site and fucose residues are excluded at the time of parasitophorous vacuole formation while Class I HLA molecules are present only on a minority of Toxoplasma-containig vacuoles
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Toxoplasma
/
Cordon ombilical
/
Endothélium vasculaire
/
Antigènes d'histocompatibilité de classe I
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2002
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Fiocruz/BR
/
Universidade Federal do Rio de Janeiro/BR
/
Universidade do Estado do Rio de Janeiro/BR
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