An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
Mem. Inst. Oswaldo Cruz
;
91(3): 279-284, May-Jun. 1996.
Article
Dans Anglais
| LILACS
| ID: lil-319872
RESUMO
Sequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Oligonucléotides
/
ADN kinétoplastique
/
Leishmania
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1996
Type:
Article
Pays d'affiliation:
Brésil
/
Panama
Institution/Pays d'affiliation:
Instituto Oswado Cruz/BR
/
Instituto Oswaldo Cruz/BR
/
Universidad de Panamá/PA
/
Universidade Federal do Rio de Janeiro/BR
/
Universidade do Estado do Rio de Janeiro/BR
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