Chimpanzees and supporting models in the study of malaria pre-erythrocytic stages
Mem. Inst. Oswaldo Cruz
;
89(Suppl.2): 111-114, 1994.
Article
Dans Anglais
| LILACS
| ID: lil-319962
RESUMO
Chimpanzees are being used in the study of immune response to Plasmodium falciparum malaria pre-erythrocytic stages (MPES). Responses induced by immunisation with recombinant/synthetic antigens and by irradiated sporozoites are being evaluated in a model system that is phylogenetically close to humans and that is amenable to limited manipulation not possible in humans. The value of chimpanzees for the in-depth study of immunological mechanisms at work in MPES-induced protection are discussed. A total number of 7 chimpanzees have been used to evaluate the immune response to recombinant antigens, and 5 have been challenged with large numbers of sporozoites, followed by surgical liver-wedge resection, in order to generate infected liver tissue for histological and immunological studies. As a complementary model, SCID mice carrying live, transplanted human and primate hepatocytes have been inoculated with sporozoites and infection of transplanted cells has been monitored by histological and immunological methods. In ongoing experiments chimpanzees are being immunised with MPES-derived lipopeptides that have been shown to overcome MHC restriction in mice, and with irradiated sporozoites.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Pan troglodytes
/
Paludisme
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1994
Type:
Article
Pays d'affiliation:
France
/
Pays-Bas
Institution/Pays d'affiliation:
Biomedical Primate Research Center-TNO/NL
/
Institut Pasteur/FR
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