En busca de secuencias retrovirales relacionadas con el cáncer de mama humano / Searching for retroviral sequences related to human breast cancer
Medicina (B.Aires)
;
57(Supl.2): 75-80, Aug. 1997.
Article
Dans Espagnol
| LILACS
| ID: lil-320007
RESUMO
The participation of viruses in mammary carcinogenesis has been largely studied in animals. A model similar to the mouse mammary tumor virus (MMTV) was previously proposed. Several lines of research supported the participation of MMTV in human breast cancer, but these evidences were contradicted when further research was performed. One major issue was the presence of human endogenous retroviral sequences that confounded results reporting MMTV-like sequences in human breast cancer. To overcome this problem we selected a 660 bp sequence of the MMTV env gene with low homology to endogenous sequences and search for a sequence to it using the polymerase chain reaction (PCR). The sequence was found in 38 of the human breast cancers and in 2 of the normal breasts studied. The sequence was not present in tumors from other organs. It was 90-98 homologous to MMTV and only 18 to human endogenous retrovirus (HERV) K-10. It was also detected in some of the positive tumors by Southern blot hybridization using one of the cloned 660 bp as a probe. Using reverse transcriptase PCR, it was possible to demonstrate that the 660 bp sequence is expressed in the majority of the tumors. Also, preliminary experiments revealed that sequences related to the LTR and gag genes of MMTV were present in the DNA of breast tumors. The origin of the MMTV-like sequences in tumor DNA could be the result of integrated MMTV-like sequences derived from a human mammary virus or may represent unknown endogenous sequences that can only be detected in breast tumors.
Recherche sur Google
Indice:
LILAS (Amériques)
Sujet Principal:
Infections à virus oncogènes
/
Tumeurs du sein
/
Virus de la tumeur mammaire de la souris
/
Infections à Retroviridae
/
Tumeurs expérimentales de la mamelle
Limites du sujet:
Animaux
/
Humains
langue:
Espagnol
Texte intégral:
Medicina (B.Aires)
Thème du journal:
Médicament
Année:
1997
Type:
Article
Pays d'affiliation:
États-Unis d'Amérique
Institution/Pays d'affiliation:
Mount Sinai School of Medicine/US
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS