Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
Mem. Inst. Oswaldo Cruz
;
97(6): 877-880, Sept. 2002. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-320142
RESUMO
American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma cruzi
/
Variation génétique
/
ADN ribosomique
/
Vecteurs insectes
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Colombie
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2002
Type:
Article
Pays d'affiliation:
Brésil
/
Colombie
Institution/Pays d'affiliation:
Centro Internacional de Entrenamiento e Investigaciones Médicas/CO
/
Fiocruz/BR
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