A simple and inexpensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
Rev. Inst. Med. Trop. Säo Paulo
;
38(3): 241-242, May-Jun. 1996.
Article
Dans Anglais
| LILACS
| ID: lil-321804
ABSTRACT
Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x 8 in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2) of samples incubated in jars with gas generator envelopes and from 129 (98) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Campylobacter
/
Techniques microbiologiques
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Rev. Inst. Med. Trop. Säo Paulo
Thème du journal:
Médecine tropicale
Année:
1996
Type:
Article
Pays d'affiliation:
Costa Rica
Institution/Pays d'affiliation:
University of Costa Rica/CR
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