IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
Biol. Res
;
35(2): 195-202, 2002. ilus
Article
Dans Anglais
| LILACS
| ID: lil-323341
RESUMO
Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including results obtained using inhibitors specific for individual steps, allowed us to connect these in a sequential manner. As the same type of signaling cascade can be triggered by oxidants, neurotransmitters and hormones, the ensemble of results allows us to propose a general model to describe signaling events that link membrane stimulation to regulation of gene transcription in skeletal muscle cells
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Canaux calciques
/
Régulation de l'expression des gènes
/
Muscles squelettiques
/
Signalisation calcique
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Biol. Res
Thème du journal:
Biologie
Année:
2002
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Chili
Institution/Pays d'affiliation:
Universidad de Chile/CL
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS