Detection of Giardia duodenalis antigen in human fecal eluates by enzyme-linked immunosorbent assay using polyclonal antibodies
Mem. Inst. Oswaldo Cruz
;
97(8): 1165-1168, Dec. 15, 2002. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-326337
ABSTRACT
The present study developed and standardized an enzime-linked immunosorbent assay (ELISA) to detect Giardia antigen in feces using rabbit polyclonal antibodies. Giardia cysts were purified from human fecal samples by sucrose and percoll gradients. Gerbils (Meriones unguiculatus) were infected to obtain trophozoites. Rabbits were inoculated with either cyst or trophozoite antigens of 14 Colombian Giardia isolates to develop antibodies against the respective stages. The IgG anti-Giardia were purified by sequential caprylic acid and ammonium sulfate precipitation. A portion of these polyclonal antibodies was linked to alkaline phosphatase (conjugate). One hundred and ninety six samples of human feces, from different patients, were tested by parasitologic diagnosis 69 were positive for Giardia cysts, 56 had no Giardia parasites, and 71 revealed parasites other than Giardia. The optimal concentration of polyclonal antibodies for antigen capture was 40 æg/ml and the optimal conjugate dilution was 1100. The absorbance cut-off value was 0.24. The parameters of the ELISA test for Giardia antigen detection were sensitivity, 100 percent (95 percent CI 93.4-100 percent); specificity, 95 percent (95 percent CI 88.6-97.6 percent); positive predictive value, 91 percent (95 percent CI 81.4-95.9 percent); and negative predictive value, 100 percent (95 percent CI 96.1-100 percent). This ELISA will improve the diagnosis of Giardia infections in Colombia and will be useful in following patients after treatment
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Anticorps antiprotozoaires
/
Giardiase
/
Fèces
/
Giardia
/
Antigènes de protozoaire
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2002
Type:
Article
Pays d'affiliation:
Bolivie
/
Colombie
/
États-Unis d'Amérique
Institution/Pays d'affiliation:
Instituto Nacional de Salud/CO
/
Ochsner Medical Foundation/US
/
Tulane University Health Sciences Center/US
/
Universidad El Bosque/CO
/
Universidad Nacional de Colombia/CO
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