Toxicity of puffer fish - two species(Lagocephalus laevigatus, LINAEUS 1766 and Sphoeroides spengleri, BLOCH 1785) from the southeastern Brazilian coast
J. venom. anim. toxins incl. trop. dis
;
9(1): 76-88, 2003. mapas, tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-329533
ABSTRACT
In Brazil, where puffer fish are considered poisonous, there are few documented cases on human consumption and consequent poisoning. In this study, toxicity of two puffer fish species from the Brazilian coast was examined. Specimens of Sphoeroides spengleri and Lagocephalus laevigatus were caught in Säo Sebastiäo Channel (North coast of Säo Paulo State, Brazil) between January 1996 and May 1997. Acidic ethanol extracts from muscle and skin plus viscera were tested for mice acute toxicity using the standard method of Kawabata. Polar extracts of S. spengleri showed high toxicity up to 946 MU/g. Extracts from L. laevigatus showed very low levels of toxicity, never exceeding 1.7 MU/g. All extracts from both species blocked amielinic nerve fiber evoked impulses of crustacean legs; this effect reverted on washing similar to the standard tetrodotoxin TTX. The aqueous extract solutions were partially purified using an ionic exchange column (Amberlit GC-50) followed by treatment with activated charcoal (Norit-A). The presence of TTX and their analogs in the semi-purified extracts were confirmed by HPLC and mass spectrometry (MALDI-TOF).
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Toxines biologiques
/
Brésil
/
Poissons venimeux
/
Maladies d'origine alimentaire
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
J. venom. anim. toxins incl. trop. dis
Thème du journal:
Toxicologie
Année:
2003
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
EMBRAPA/BR
/
University of Brasilia/BR
/
University of Säo Paulo/BR
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