Microsporogénesis y microgametogénesis de onoto (Bixa orellana L. ) / Microsporogenesis and microgametogenesis of annatto (Bixa orellana L. )
Acta cient. venez
;
53(3): 171-175, 2002. ilus
Article
Dans Espagnol
| LILACS
| ID: lil-331334
ABSTRACT
A series of buds of increasing maturity were individually sampled in order to examine cytological events of annatto (Bixa orellana L.), genotype Portuguesa. They were fixed in Carnoy II at 1230 am, time of the highest rate of meiotic division. Three stain solutions were attempted. In the microspores mother cells, the use of acetic orcein 1% resulted in a good nucleus coloration and sharpness. In contrast, a well chromosome resolution was achieved with the application of propionic carmin 2%. The pollen grain mother cells (n = 8 chromosomes) at metaphase I were found in floral buds of 0.5 to 0.6 cm long; tetrad stage in buds of 0.6 to 0.7 cm long, uninucleate stage of microspores in buds of 0.7 to 0.8 cm long and the binucleate stage (pollen) in buds longer than 0.8 cm. Microphotographies showing the sequence of meiotic division (microsporogenesis) and subsequent mitosis to originate pollen grains were included
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Pollen
/
Division cellulaire
/
Chromosomes
/
Bixaceae
langue:
Espagnol
Texte intégral:
Acta cient. venez
Thème du journal:
Science
Année:
2002
Type:
Article
Pays d'affiliation:
Venezuela
Institution/Pays d'affiliation:
Universidad Central de Venezuela/VE
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