Criteria for Candida albicans numerical analysis based on electrophoretic protein patterns
Rev. argent. microbiol
;
32(3): 123-128, jul.-sept. 2000.
Article
Dans Anglais
| LILACS
| ID: lil-332526
ABSTRACT
Even in normalized gels, some problems with the choice and position of protein bands always have hindered the processing of electrophoretic data. We have developed a way to establish which criterion best fits the necessities in order to maximize the similarity indexes for numerical analysis. Some repetitions of a Candida albicans strain were carried out in eleven different gels. After staining, the bands were scored in numbers within ranges of +/- n values with increases of one quarter steps (+/- 0.25 kDa, +/- 0.5 kDa, +/- 0.75 kDa, +/- 1.0 kDa, +/- 1.25 kDa) that will limit the possibility of variation for the same bands that will appear on the repetitions. Using this criterion, we have determined that values scored within +/- 1.25 kDa could optimize the minimum limiting value of similarity in different dendrograms built.
Recherche sur Google
Indice:
LILAS (Amériques)
Sujet Principal:
Candida albicans
/
Protéines fongiques
/
Classification
/
Densitométrie
/
Électrophorèse sur gel de polyacrylamide
/
Mycologie
langue:
Anglais
Texte intégral:
Rev. argent. microbiol
Thème du journal:
Microbiologie
Année:
2000
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
State University of Campinas/BR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS